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. 2019 Jan 10;14(1):e0210389. doi: 10.1371/journal.pone.0210389

Fig 4. Melanotan-II did not significantly impact exploratory behavior or marble burying behavior, but caused significant weight loss in C57BL/6J mice.

Fig 4

(a) Open field anxiety test quantified as number of gridlines crossed, with results shown for normal C57BL/6J mice pre- and post-vehicle control or MT-II treatment for 9 days (vehicle, n = 7; MT-II treatment, n = 9). ANOVA comparison between normal C57BL/6J mice treated with vehicle versus MT-II did not show a significant treatment interaction in regards to number of gridlines crossed; F(3, 28) = 0.89. Sidak’s multiple comparison tests did not show a significant change in open field anxiety test performance in the vehicle treated mice (p = 0.97) or MT-II treated mice (p = 0.73). (b) Marble burying testing quantified as the percentage of marbles buried, with results shown for normal C57BL/6J mice pre- and post-vehicle control or MT-II treatment for 14 days (vehicle, n = 7; MT-II treatment, n = 7). ANOVA comparison between normal C57BL/6J mice treated with vehicle versus MT-II did not show a significant treatment interaction with regards to marble burying; F(3, 24) = 0.067; p = 0.97. Sidak’s multiple comparison tests did not show a significant change in marble burying in the vehicle treated mice (p = 0.97) or MT-II treated mice (p = 0.94). (c) Weight in grams shown for normal C57BL/6J mice pre- and post-vehicle control or MT-II treatment for 14 days (vehicle, n = 7; MT-II treatment, n = 7). ANOVA comparison between normal C57BL/6J mice treated with vehicle versus MT-II did not show a significant treatment interaction with regards to weight loss; F(3, 24) = 2.35; p = 0.098. Sidak’s multiple comparison test showed no significant change in the vehicle treated mice; p = 0.97. Sidak’s multiple comparison test showed significant weight loss in the MT-II treated mice; p = 0.037. (a-c): data represented as mean +/- SEM.