Table 2. All possible beneficial mutations for scFv protein that improves its binding to FuBc.
| Mutation | Mutation Energy | Mutation | Mutation Energy | Mutation | Mutation Energy |
|---|---|---|---|---|---|
| THR28.GLN | -0.852045 | THR59.VAL | -1.06502 | SER227.TRP | -3.2534 |
| THR30.TRP | -1.90106 | THR59.ILE | -1.05671 | SER227.LEU | -0.86066 |
| THR30.PHE | -1.38101 | THR59.CYS | -0.90064 | SER227.ARG | -0.741184 |
| THR30.ILE | -1.37215 | THR59.PHE | -0.65846 | SER227.CYS | -0.64038 |
| THR30.LEU | -1.12846 | THR59.TRP | -0.54201 | SER227.MET | -0.61869 |
| THR30.CYS | -1.08166 | GLU61.TRP | -1.84329 | SER227.GLN | -0.57443 |
| THR30.VAL | -0.88388 | GLU61.PHE | -1.52833 | PRO229.MET | -1.30307 |
| THR30.TYR | -0.859165 | GLU61.TYR | -0.801355 | PRO229.GLN | -1.24191 |
| ASP31.PHE | -3.02031 | GLU61.ARG | -0.72688 | PRO229.LYS | -1.05112 |
| ASP31.ARG | -2.34575 | GLU61.MET | -0.63501 | PRO229.CYS | -1.01353 |
| ASP31.ILE | -1.81088 | GLU61.GLN | -0.557085 | PRO229.ALA | -0.78185 |
| ASP31.LYS | -1.7751 | GLY103.THR | -0.844785 | HIS230.PHE | -2.30479 |
| ASP31.GLN | -1.66762 | GLY103.CYS | -0.757655 | HIS230.TRP | -2.30271 |
| ASP31.MET | -1.23667 | TYR105.TRP | -1.87079 | HIS230.TYR | -1.93659 |
| ASP31.TYR | -1.15606 | TYR105.PHE | -1.44166 | HIS230.LEU | -1.84049 |
| ASP31.LEU | -1.14876 | TYR107.TRP | -1.13285 | HIS230.ILE | -1.21832 |
| ASP31.CYS | -1.09831 | SER165.TRP | -0.8701 | HIS230.ARG | -0.841608 |
| ASP31.THR | -1.09109 | SER165.TYR | -0.819385 | HIS230.GLU | -0.696465 |
| ASP31.ASN | -1.06624 | SER165.CYS | -0.657575 | HIS230.VAL | -0.62586 |
| ASP31.HIS | -0.855045 | SER165.GLN | -0.61451 | HIS230.MET | -0.57906 |
| ASP31.VAL | -0.81338 | SER165.ARG | -0.564398 | HIS230.SER | -0.56195 |
| ASP31.GLU | -0.63503 | TYR166.TRP | -1.21901 | HIS228.ASN | -0.532915 |
| TYR32.TRP | -1.51204 | SER226.GLN | -1.14458 | ||
| TYR32.PHE | -0.52085 | SER226.TYR | -1.06741 | ||
| TYR33.GLN | -0.939895 | SER226.THR | -0.59387 | ||
| TYR33.LEU | -0.883955 | SER226.CYS | -0.51582 |