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. 2019 Jan 10;14(1):e0209576. doi: 10.1371/journal.pone.0209576

Table 3. Best stabilizing triple mutation combination for scFv with their mutation energy.

Sr. No. Mutation Mutation Energy
1 THR30.TRP,TYR105.TRP,SER227.LEU -9.57361
2 THR30.TRP, ASP31.GLN,SER227.TRP -9.23207
3 THR30.TRP,ASP31.GLN,SER227.LEU -8.9884
4 THR30.TRP,ASP31.LEU,SER227.LEU -8.61851
5 THR30.TRP,ASP31.LEU,SER227.TRP -8.576
6 THR30.TRP,ASP31.THR,SER227.LEU -8.55091
7 THR30.TRP,GLY103.THR,SER227.TRP -8.42681
8 TYR33.GLN,GLY103.THR,SER227.LEU -8.3736
9 TYR33.GLN,GLY103.THR,SER227.TRP -8.34917
10 TYR32.TRP,THR30.PHE,SER227.LEU -8.07606
11 THR30.TRP,SER227.LEU,HIS230.TRP -7.7884
12 TYR105.TRP,ASP31.LEU,SER227.TRP -7.70835
13 THR30.TRP,TYR105.TRP,SER227.TRP -7.61662
14 THR30.TRP,GLY103.THR,SER227.LEU -7.387
15 THR30.TRP,ASP31.LEU,HIS230.PHE -7.3348
16 THR30.TRP,TYR32.TRP,SER227.LEU -7.20526
17 TYR105.TRP,ASP31.LEU,SER227.LEU -7.15371
18 ASP31.LEU,SER227.LEU,HIS230.TRP -7.08499
19 TYR32.TRP,THR30.PHE,SER227.TRP -7.07932
20 TYR105.TRP,SER227.LEU,HIS230.TRP -7.02525