Skip to main content
. 2019 Jan 10;14(1):e0210193. doi: 10.1371/journal.pone.0210193

Fig 1. HNK-1 epitope is expressed on the N-glycan of tenascin-C.

Fig 1

(A) Soluble fractions prepared from embryonic day 16 (E16), postnatal day 0 and 7 (P0 and P7, respectively), 2- and 10-week-old (2W and 10W, respectively) mouse brains were immunoblotted with HNK-1 mAb, anti-TNC pAb, and anti-Tubulin mAb (for loading control). Each blot was representative of 12 images of western blots. 50, 80, and 20 μg proteins were loaded to each lane for HNK-1, TNC, and Tubulin, respectively. (B) Soluble fractions of E16, P0, P7, and 2W mouse brains were immunoprecipitated with anti-TNC pAb and antibody-bound (Bound) and unbound (Unbound) fractions were obtained. Each blot was representative of 8 images of western blots. 200 μg proteins were used for each immunoprecipitation. (C) Soluble fractions of E16, P0, P7, and 2W mouse brains were treated with or without peptide N-glycosidase F (PNGase F or No treatment, respectively). The blot was representative of 5 images of western blots. 20 μg proteins were loaded to each lane.