Figure 1. Pgc Is Translationally Regulated via Its UTRs.

(A) Schematic representation of a Drosophila ovariole.

(B) Schematic representation of a germarium housing the germline stem cells (GSCs) (blue), pre-cystoblast (pre-CB) (green), and differentiating cysts. The singlecells of the germarium can be identified by spectrosomes and the differentiating cysts can be identified by fusomes.

(C) The ovariole of a pgcGFP ovary stained with 1B1 (red), which marks the spectrosomes and fusomes; Vasa (blue), which marks the germline; and GFP (green), which marks Pgc-expressing cells. Expression of GFP is restricted to the pre-CB (arrow).

(D) The ovariole of a wild-type fly probed for pgc RNA (magenta) using FISH shows that pgc RNA is present throughout oogenesis.

(E) RNA-seq track of pgc in nosGAL4 > UAS-tkv ovaries.

(F) The ovariole of a transgenic fly (pgc promoter-nos 5′ UTR-GFP-K10 3′ UTR) stained with 1B1 (red), Vasa (blue), and GFP (green). GFP expression shows that pgc promoter is active throughout oogenesis (dashed line).

(G) The ovariole of a transgenic fly (pgc promoter-nos 5′ UTR-GFP- pgc 3′ UTR) stained with 1B1 (red), Vasa (blue) and GFP (green) shows GFP expression only in the earliest stages of oogenesis (dashed line).

(H) The ovariole of a transgenic fly (pgc promoter-pgc 5′ UTR-GFP-K10 3′ UTR) stained with 1B1 (red), Vasa (blue), and GFP (green) shows GFP expression throughout oogenesis (dashed line).

Scale bars, 10 μm. See also Figure S1.