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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: Cell Rep. 2018 Dec 26;25(13):3828–3843.e9. doi: 10.1016/j.celrep.2018.12.007

Figure 5. Pum and Its Cofactor, Brat, Regulate Pgc Translation in 4- to 16-Cell Cysts.

Figure 5.

(A) A germarium of a pgcGFP ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows expression of GFP in the pre-CB (dashed circle).

(B) A germarium of a pgcGFP; pum680 ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows aberrant expression of GFP in the differentiating cysts (25% in the 4-cell cyst, 75% in the 8-cells cyst, and 10% in the 16-cell cyst, n = 20) (dashed outline).

(C) A germarium of a pgcGFP; nosGAL4>bratRNAi ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows aberrant expression of GFP in the differentiating cysts (38% in the 4-cell cyst, 54% in the 8-cells cyst, and 18% in the 16-cell cysts, n = 30) (dashed outline).

(D) A germarium of a pgcGFP; nosGAL4>d4EHPRNAi ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows aberrant expression of GFP in the differentiating cysts (34% in the 4-cell cyst, 62% in the 8-cells cyst, and 15% in the 16-cell cyst, n = 32) (dashed outline). The GFP channel is shown in A1–D1.

(E) A developmental profile of GFP expression when the Pum-Brat interaction is ablated and Brat and d4EHP are depleted in the germline.

Scale bar, 10 μm. See also Figure S5.