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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: Cell Rep. 2018 Dec 26;25(13):3828–3843.e9. doi: 10.1016/j.celrep.2018.12.007

Figure 6. Bru and Cup Regulate Pgc Translation in the Later Stages of Oogenesis.

Figure 6.

(A) An ovariole of a pgcGFP ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows expression of GFP in the pre-CB (arrow).

(B) An ovariole of a pgcGFP; nosGAL4>bruRNAi ovary stained with 1B1 (red), Vasa (blue), and GFP (green) aberrant expression of GFP beyond the 16-cell cyst (12% from 8-cell cyst onward, 100% from 16-cell cyst onward, n = 25) (dashed outline).

(C) An ovariole of a pgcGFP; nosGAL4>cupRNAi ovary stained with 1B1 (red), Vasa (blue), and GFP (green) shows aberrant expression of GFP from the later cyst stages (20% from 8-cell cyst onward, 100% from 16-cell cyst onward, n = 30) (dashed outline). The GFP channel is shown in A1–C1.

(D) A developmental profile of GFP expression when Bru and Cup are depleted in the germline.

(E) PAT assay analysis of pgc poly(A)-tail length of pgc RNA when Bru and Cup are depleted in the germline.

Scale bars, 10 μm. See also Figure S6.