Fig 4. Relative mRNA level of different UCP isoforms in control and harpin-treated Arabidopsis thaliana cells.

Arabidopsis suspension cells were treated with HrpZ_pto at the final concentration of 150 nM. At the indicated time points, samples were taken, and total RNA was extracted. The quantitative RT-PCR was carried out by using specific primers designed for the coding sequences of UCP1, UCP2, UCP3, UCP4, UCP5 and mitosis protein YLS8 (housekeeping gene) genes as described in Materials and methods. The gene expression of the samples collected from each cell culture before treatments, was regarded as 1. Data are expressed as means ± SD from three independent HrpZ_pto treatments. (Asterisk) Significant difference with respect to control (P<0.05).