(A) Example recordings from a stomatogastric ganglion experiment with an acid-first protocol. Intracellular recordings of the PD and LP neurons and extracellular recordings of the lvn are shown. Each colored box displays 5 s of recordings taken from the last minute at each pH step. The experiment can be read left to right then top to bottom in chronological order. Horizontal lines indicate a reference membrane potential of −40 mV; vertical lines indicate a scale of 10 mV. (B) Pyloric frequency, (C) number of PD spikes per burst, and (D) PD duty cycle were calculated for the last 8 min of each pH step. Violin plots show the KDE distribution, mean, median, IQR, and 95% CI for each measure across pH conditions. Recoveries from acid and base are displayed in the shaded gray regions on the far ends of each plot.
Figure 2—source data 1. Numerical data represented in panel B, C, and D of Figure 2.