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. 2018 Dec 28;7:e41877. doi: 10.7554/eLife.41877

Figure 4. Intracellular characteristics of semi-isolated pyloric neurons, PD and LP.

Several characteristics of the PD and LP neurons in the presence of picrotoxin (PTX) were measured for the last minute of each pH condition. (A) Example intracellular recordings of PD and LP neurons prior to PTX application and in the presence of PTX across pH conditions. Horizontal lines indicate a reference membrane potential of −40 mV; the vertical line indicates a scale of 10 mV. (B,C) Minimum membrane potential and (D,E) spike amplitude are plotted for LP and PD as a function of pH. (F) PD burst frequency and (G) LP firing rate are also plotted at each pH. Violin plots show the KDE distribution, mean, median, IQR, and 95% CI for each measure across pH conditions. Recoveries from acid and base are displayed in the shaded gray regions on the far ends of each plot. Asterisks denote statistical significance revealed by paired samples t-tests with Bonferroni correction (*p<0.05; **p<0.01; ***p<0.001).

Figure 4—source data 1. Numerical data represented in panel B, C, D, E, F, and G of Figure 4.
elife-41877-fig4-data1.zip (35.6KB, zip)
DOI: 10.7554/eLife.41877.011

Figure 4.

Figure 4—figure supplement 1. Statistical analysis of the effects of pH on semi-isolated PD and LP neurons.

Figure 4—figure supplement 1.

The main effects of acid and base protocols on six measures of the activity of semi-isolated LP and PD neurons were assessed. Univariate Type III Repeated-Measures Analysis of Variance (ANOVA) tests were performed separately for both acid and base step protocols. Post-hoc paired samples t-tests with Bonferroni correction were performed for each pH step against its respective control, the pH 7.8 condition immediately prior to the step protocol. T-statistics are not reported for non-significant ANOVAs.