Figure 3.

(a) [³⁵S]GTPγS binding upon stimulation of U2OS-CCR1 and U2OS-CCR2 by increasing concentrations of CCL3 and CCL2, respectively. In both cases, the response was corrected by subtracting the basal activity (approximately 8000 dpm for both CCR1 and CCR2). (b) Inhibition of CCL3-induced [³⁵S]GTPγS binding by compounds 39, 41, 43, and 45 in U2OS-CCR1. (c) Inhibition of CCL2-induced [³⁵S]GTPγS binding by compounds 39, 41, 43, and 45 in U2OS-CCR2. The level of basal activity in U2OS-CCR1 and U2OS-CCR2 is indicated by a dashed line. In all cases, data shown are mean ± SEM of at least three experiments performed in duplicate.