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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: Nat Protoc. 2012 Dec 6;8(1):33–42. doi: 10.1038/nprot.2012.143

Figure 3|.

Figure 3|

Co-staining strategies to improve the identification and isolation of human TSCM cells. (a) Human CD4+ and CD8+ naive-like cells were identified as described previously4. The expression of CCR7 (left), CD58 (center) or CD122 (right) versus CD95 in human CD4+ and CD8+ T cells is shown. The gate identifies TSCM cells as depicted in Figure 1b. Numbers indicate the percentage of cells identified by the gates. (b) Expression of CD95 by CD4+ and CD8+ TSCM cells identified by the increased expression of CD58 and CD122. T cell subsets are defined as in Figure 1. Memory T cells are defined as positive for CD45RO.