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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: J Clin Immunol. 2014 Dec 13;35(2):112–118. doi: 10.1007/s10875-014-0116-2

Fig. 2. Validation of novel mutation in MAGT1.

Fig. 2

a RT-PCR showing the level of MagT1 mRNA normalized to GAPDH in PBMCs from the nephew compared to three controls. All samples were assayed on at least two different days and each time in duplicate or triplicate.Asterisks indicate P<0.02. b Flow cytometry of NKG2D surface expression on CD8 cells from the nephew and normal controls. c Intracellular magnesium level from then nephew and normal controls. Flow cytometry shows the ratio of the mean fluorescent intensity (MFI) of a Mg2+-specific fluorescent probe (MagFluo4) to the MFI of a Ca2+-sensitive probe (FuraRed) as (MF4/FR) in PBMCs. The ratio is set at 1 for the normal controls. Isotype control antibody staining indicates background fluorescence in both panels. All plots are representative of at least 2 independent experiments.