Fig. 2.

E200-targeted antibodies specifically bind nfP2X₇. a, b ELISA assay of BIL03s (a) and BPM09 (b) binding to E200 peptide compared with PBS and isotype controls. Results were normalised to maximum binding. Mean and SEM from three independent experiments are shown. c Quantification of P2RX7 transcript expression relative to a panel of housekeeping genes in PC3 cells 72 h after transfection with P2RX7-targeted siRNA. Mean and SEM from four independent experiments are shown. Each experiment was normalised to untreated control. One-way ANOVA with Dunnett’s post test was used to test significance. d BIL03s binding to live PC3 cells 72 h after transfection with P2RX7-targeted siRNA was tested by flow cytometry. Mean and SEM from four independent experiments are shown. Two-way ANOVA with Dunnett’s post test was used to test significance. e BIL03s binding to live PC3 cells, measured by flow cytometry, can be competed by E200 peptide. Mean and SEM from three independent experiments are shown. One-way ANOVA with Dunnett’s post test was used to test significance. f Flow cytometry analysis shows that BPM09 binding to live PC3 cells can be competed with increasing amounts of BIL03s. Representative plot from three experiments are shown. g Representative flow cytometry plots from three experiments showing BIL03s binding to live cells in a panel of cancer cell lines. *P < 0.05, **P < 0.01