Fig. 6.

nfP2X₇ is necessary for cancer cell survival. a, b PC3, DU145 and LNCaP non-functional cell lines were transfected with P2RX7-targeted siRNA. After 72 h, a number of live cells were measured using CellTitre-Blue (CTB) assay. b Induction of apoptosis was measured using an Apo One caspase 3/7 activation assay. Mean and SEM from at least four independent experiments shown. One-way ANOVA with Dunnett’s post test was used to test significance. c, d SK-MEL-5 functional cells were transfected with P2RX7-targeted siRNA. After 72 h, c no effect on cell viability was measured by CTB assay. d Surface expression of P2X₇ as measured by L4 flow cytometry binding was significantly reduced. Mean and SEM from at least four independent experiments shown. Two-way ANOVA with Dunnett’s post test was used to test significance. e PC3 cells or PC3 cells overexpressing P2X₇a were transfected with P2RX7-targeted siRNA. After 72 h, number of live cells was quantified using a CTB assay. P2RX7-targeted siRNA caused a reduction in cell numbers even in the presence of overexpressed P2X₇a. Mean and SEM from at least four independent experiments shown. Two-way ANOVA with Dunnett’s post test was used to test significance. *P < 0.05, **P < 0.01, ***P < 0.001