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. 2019 Jan 4;12:498. doi: 10.3389/fncel.2018.00498

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Copyright © 2019 Xu, Xing, Huang, Zhou, Liu, Cui, Cheng, Wang, Zhou, Yang, Yang, Zhang, Zang, Ma and Guan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and expression of histone deacetylase 1 (HDAC1) in hUC-MSCs. (A) Wharton’s jelly tissue pieces (red arrow) were plated and cultured to allow primary hUC-MSCs (P0) to grow out. (B) MSCs after 2 weeks of primary culture (P0). (C) P3 hUC-MSCs, Scale bar = 100 μm. (D) Immunophenotypic characterization of hUC-MSCs by flow cytometry. (E) Relative HDAC1 mRNA expression by quantitative real-time-PCR (qRT-PCR). (F) Representative expression of HDAC1 by Western blotting and (G) densitometric analysis of HDAC1 protein. Data are presented as mean ± SEM. *p < 0.05 vs. MSCs, ^#p < 0.05 vs. MSCs-siCON (MSCs transfected with silencing lentivirus control).