Figure 5.

Analysis of the frequency of CD69, TNF-α, and IL-10 in CD4, CD8, and double-negative (DN/CD4⁻CD8⁻) cells expressing gamma-delta TCR. (A) Representative dot plots and histograms illustrating the gating strategy to access frequency of expression of cytokines TNF-α and IL-10 in TCR gamma-delta⁺ cells (B) Frequency of CD4⁺TCRγδ⁺CD69⁺ lymphocytes in non-stimulated cultures. (C) Frequency of CD8⁺TCRγδ⁺CD69⁺ lymphocytes. (D) Frequency of CD4⁻CD8⁻TCRγδ⁺CD69⁺ lymphocytes. (E) Frequency of CD4⁺TCRγδ⁺TNF-α⁺ lymphocytes. (F) Frequency of CD8⁺TCRγδ⁺TNF-α⁺ lymphocytes. (G) Frequency of CD4⁻CD8⁻TCRγδ⁺TNF-α⁺. (H) Frequency of CD4⁺TCRγδ⁺IFN-γ⁺. (I) Frequency of CD8⁺TCRγδ⁺ IFN-γ⁺. (J) CD4⁻CD8⁻TCRγδ⁺ IFN-γ⁺. (K) Frequency of CD4⁺TCRγδ⁺IL-10⁺. (L) Frequency of CD8⁺TCRγδ⁺IL-10⁺. (M) CD4⁻CD8⁻TCRγδ⁺IL-10⁺ White box plots represent non-stimulated cultures and dark gray box plots represent PRO stimulated cultures from non-infected individuals (NI), indeterminate Chagas patients (I) and cardiac Chagas patients (C). (C) The results are expressed as percentage in box plot format. The box extends from the 25th percentile to 75th percentile, with a horizontal line at the median (50th percentile). Whiskers extend from the lowest value to the 25th percentile and from the 75th percentile to the highest value, showing the range of data distribution. Statistical significance is indicated in each graph.