Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 4;8:655. doi: 10.3389/fonc.2018.00655

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Yu, Tang, Lin, Yao and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Lentivirus-mediated DGKZ knockdown specifically inhibited DGKZ expression in osteosarcoma cells: Saos-2, HOS, and U2OS infected with DGKZ -shRNA or shCtrl lentivirus and non-transfected cells examined by fluorescent microscopy and light microscopy at the 3rd day after infection. More than 80% of cells expressed GFP. There was no significant difference between the negative control group and the non-transfected group, indicating the transfection process has no effect on cells growth. (A–C) Saos-2, 100 × B; (D,E) Saos-2, 100 × G; (F–H) U2OS, 100 × B; (I,J) U2OS, 100 × G; (K–M) HOS, 100 × B; (N,O) U2OS, 100 × G; (P–R) Efficient knockdown of DGKZ in osteosarcoma cell lines. Saos-2 (P),U2OS (Q), and HOS (R) cells were transduced with lentivirus expressing control or DGKZ shRNA. The knockdown efficiencies were detected with qPCR.