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. 2019 Jan 4;9:1902. doi: 10.3389/fpls.2018.01902

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Copyright © 2019 Liu, Xiang, Zhang, Han, Sun, Sheng, Yan, Scheller and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alignment and phylogenetic analysis of ZmNAGK with NAGKs from different species. (A) ZmNAGK encodes a protein of 345 amino acid residues and contains an AA-kinase domain. (B) The amino-acid sequences of NAGKs from Arabidopsis thaliana, Brachypodium distachyon, Nicotiana tabacum, Oryza sativa, Setaria italica, Sorghum bicolor, and Zea mays were aligned. Identical and similar residues were shaded in black and gray, respectively. The putative plastid-target sequence, which was predicted by the iPSORT (http://ipsort.hgc.jp/) programs are boxed. (C) Phylogeny tree of NAGKs was calculated in the MEGA 6.0 program. The phylogenetic relationship between the examined sequences was performed using the Neighbor-Joining method with p-distance correction. Bootstrap values were derived from 1000 replicate runs. The accession numbers: AtNAGK (NP_191315.1), BdNAGK-like (XP_003570025.1), NtNAGK-like (XP_016482712.1), OsNAGK1 (XP_015633506.1), OsNAGK2 (BAD88532.1), SiNAGK-like (XP_004976436.1), SbNAGK-like (XP_002446901.2), ZmNAGK (XP_008668008.1).