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. 2019 Jan 10;9:30. doi: 10.1038/s41598-018-37188-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxic potential of targeted therapeutic PLX4032 in BRAF^V600E melanoma cells T24.6.9 is considerably higher than for classical chemotherapeutics. (A) Impedimetric detection of concentration- and time-dependent chemosensitivity to dacarbazine, cisplatin and PLX4032. Values are normalised to experiment starting point and solvent control (dashed line, 100%). (B) Linear fitting and time-dependent linear progression of logarithmised IC₅₀ values with 95% confidence intervals (dashed lines) assessed by impedance spectroscopy (upper panel) and XTT assay (lower panel). (C) Staining of 5-ethynyl-2′-deoxyuridine (EdU)-positive (green), proliferating cells in untreated and treated cultures (3000 µM Dacarb, 10 µM cisPt or 10 µM PLX4032) after 72 h (red: MelanA, blue: cell nuclei, bar = 50 µm). (n values depicted in figure; mean ± s.e.m.; **P < 0.01; ***P < 0.001); Dacarb = dacarbazine; cisPt = cisplatin; XTT = tetrazolium salt-based cytotoxicity assay.