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. 2019 Jan 10;9:31. doi: 10.1038/s41598-018-37251-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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lncRNA gene BLNCR is located nearby epidermal stem cell marker ITGB1 and is transcribed independently. (a) Upper panel: Publicly available data of RNA-sequencing show expression of both transcripts (Kretz, M. et al., 2012 (GSE35468)) and histone marks (NHEK tracks, ENCODE). Lower panels: Expression of ITGB1 and BLNCR during differentiation. n = 3, mean −/+ SD. Statistical significance was assessed using a t-test: *p < 0,05; **p < 0,01; ***p < 0.001. (b) Detection of RT-product by qPCR of ITGB1 and BLNCR after strand-specific RT-qPCR. n = 3, mean −/+ SD. (c) Relative expression of ITGB1 and BLNCR after 48 h knockdown of ITGB1. Percentage knockdown ITGB1: 96%, n = 3, mean −/+ SD. Statistical significance was assessed using a t-test: *p < 0,05; **p < 0,01; ***p < 0.001.