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. 2019 Jan 10;9:31. doi: 10.1038/s41598-018-37251-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BLNCR expression is regulated by EGF signaling and downregulation precedes the point-of-no-return in differentiation. (a) Relative 18s normalized expression of ITGB1 and BLNCR in a time-course experiment with AG1478 treatment. Data on BLNCR is already visualized in Fig. 2. n = 3, mean −/+ SD. Statistical significance was assessed using a t-test: *p < 0,05; **p < 0,01; ***p < 0.001. (b) Upper panel: Overview of the assay set-up. Lower panels: Number of colonies and colony area. Cells were treated with 10 μM AG1478 for the indicated times before they were seeded in the CFA. n = 3.