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. 2019 Jan 10;10(1):31. doi: 10.1038/s41419-018-1241-2

Fig. 2. The PI3K/Akt pathway is involved in resistin-induced VEGF-A expression in human chondrosarcoma cells.

Fig. 2

a, b Cells were pretreated for 30 min with Ly294002 (10 μM), wortmannin (5 μM), and an Akt inhibitor (10 μM), or transfected with p85 or Akt siRNA then stimulated with resistin. The VEGF-A expression was examined by qPCR and ELISA. c JJ012 cells were incubated with resisitin, the p-p85 and Akt expression was examined by Western blot. d JJ012 cells were pretreated with Ly294002 for 30 min, then stimulated with resistin and Akt phosphorylation was examined. e JJ012 cells were transfected with p85 or Akt siRNA, the p85 and Akt expression was examined. Results are expressed as the mean ± SEM. *p < 0.05 as compared with the control group; #p < 0.05 as compared with the resistin-treated group; +p < 0.05 as compared with the control siRNA group