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. 2019 Jan 10;10(1):31. doi: 10.1038/s41419-018-1241-2

Fig. 3. Resistin promotes VEGF-A and angiogenesis via inhibiting miR-16-5p.

Fig. 3

a, b Cells were incubated with resistin for 24 h and the indicated miRNAs expression was examined by qPCR. c, d Cells were transfected with the miR-16-5p mimic then incubated with resisin for 24 h; VEGF-A expression was measured by qPCR and ELISA. e–g The CM was applied to EPCs and analyzed for tube formation and migration activity (Size bar = 200 μm). h, i The CM also applied to chick embryos for 4 days and then resected, fixed, and photographed with a stereomicroscope (Size bar = 1 mm). j, k Cells were treated with PI3K and Akt inhibitors or siRNAs then incubated with resisin for 24 h; The miR-16-5p expression was measured by qPCR. l VEGF-A-3′UTR luciferase plasmids contain the miR-16-5p binding site. m, n Cells were treated with PI3K and Akt inhibitors or siRNAs then incubated with resisin for 24 h; The VEGF-A luciferase activity was measured. Results are expressed as the mean ± SEM. *p < 0.05 as compared with the control group; #p < 0.05 as compared with the resistin-treated group; +p < 0.05 as compared with the control siRNA group