Fig. 5. Overexpression of resistin induces VEGF-A-dependent angiogenesis by inhibiting miR-16-5p in vivo.

a, b Mice (n = 10 each group) were injected subcutaneously with the indicated cells. At 14 days after injection, the tumors were excised, photographed with a microscope, and hemoglobin levels were measured. c Tumor sections were immunostained with resistin, VEGF-A, CD31, CD34, and CD133 antibodies (Size bar = 100 μm) d Matrigel plugs were treated with indicated CM and subcutaneously injected into the flanks of nude mice. After 7 days, the plugs were photographed and hemoglobin levels were examined. e The indicated CM was applied to chick embryos for 4 days then resected, fixed, and photographed with a stereomicroscope (Size bar = 1 mm) (n = 7). Results are expressed as the mean ± SEM. *p < 0.05 as compared with the control group; #p < 0.05 as compared with the resistin-treated group