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. 2019 Jan 10;10:115. doi: 10.1038/s41467-018-07918-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ANO1 is localised in the zebrafish skeletal muscle surface membrane. Representative images of normal zebrafish myotubes double immunolabelled for DHPRα_1S subunit (anti-α_1S) and ANO1 (anti-ANO1) or ANO5 (anti-ANO5) isoforms showing their respective subcellular distribution. a ANO1 is expressed in the sarcolemma (centre image) and does not co-localise with DHPRα_1S (left image) in the triads (merged image). Scale bar, 5 µm. Fluorescence intensity profile (bottom) obtained by measuring the average fluorescence intensity along a horizontal line on the merged image demonstrates regular t-tubular periodicity of the DHPRα_1S (red) but a complete lack of a structured ANO1 signal (green). b Double immunofluorescence labelling of DHPRα_1S (left image) and ANO5 (centre image) under similar conditions clearly revealed anti-ANO5 signal on both sides of the t-tubular membrane (merged image) and thus indicating SR membrane localisation of ANO5. Fluorescence intensity profile displays distinct but regular spatial periodicity with one red peak (DHPR signal) flanked by two green peaks (ANO5 signal)