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. 2019 Jan 10;9:19. doi: 10.1038/s41598-018-37221-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cx43 mutations induce large hemichannel currents in Xenopus oocytes. Single cells were clamped at a holding potential of −40 mV and subjected to voltage pulses ranging from −30 to +60 mV in 10 mV steps. H₂O (a) and wild-type Cx43 (b) injected cells displayed negligible membrane currents. Cx43-G8V (c), Cx43-A44V (d), and Cx43-E227D (e) expressing oocytes displayed much larger hemichannel currents than wild-type Cx43. (f) Steady-state currents from each pulse were plotted as a function of membrane voltage. Steady state currents in wild-type Cx43 (filled squares) or H₂O injected control cells (filled circles) were negligible at all tested membrane voltages. Cx43-G8V (open squares) expressing cells exhibited significantly increased steady-state currents at all voltages compared to either control or wild-type Cx43 oocytes. Cx43-A44V (open circles), or Cx43-E227D (open triangles) currents were similar to those observed in control cells at negative voltages, but increased at positive potentials. Data are the mean ± SEM.