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. 2019 Jan 10;9:19. doi: 10.1038/s41598-018-37221-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative examples of patch clamp recordings from cell-attached patches containing single Cx43-G8V (a), Cx43-A44V (b), and Cx43-E227D (c) hemichannels in symmetric 140 mM KCl solutions. Single hemichannel currents were recorded in response to 8-s voltage ramps between −70 and +70 mV. Current-voltage relations for Cx43-A44V and Cx43-E227D were linear, whereas those of Cx43-G8V showed slight inward rectification. All three mutations show closing transitions to subconductance states. Occasional transitions to the fully closed state are also seen (b). (d) Cell-attached patches from wild-type Cx43 injected oocytes failed to show single channel activity. (e) Mean slope conductances measured at V_m = 0 are similar for the three mutant hemichannels. Values represent the mean ± SEM from 5 to 7 recordings.