Table 2.

Damage Score Criteria for Liver (Confocal Imaging).

Morphology Score	Criteria
No/Mild Damage	Liver diameter up to ~200–250 μm with clearly defined organ margin. Hepatocyte diameter ~15 μm with uniform size distribution. At least 90% of cells maintain cell-cell contacts. Sinusoids are clear as spare regions flanked by intact hepatocytes. Occasional cytoplasmic clearing.
Moderate Damage	Loss of overall liver shape and distinct margins. Increased hepatocyte size. Sinusoids are hardly visible, and enlarged cells frequently show regions of cytoplasmic clearing. Loss of visible cell contacts in 10–65% of hepatocytes (rounded appearance). Appearance of “puffed” cell bodies that probably present early stages of cell death.
Severe Damage	Strongly reduced liver size and mTq2 fluorescence. Fewer than 40% of hepatocytes are intact. At least half of cells/corpses have “puffed” phenotype. Occasionally, liver margins are hard to discern.