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. 2019 Jan 10;10:112. doi: 10.1038/s41467-018-07998-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — WTX loss promotes CRC cells, migration and liver metastasis. a Percentage of WTX IHC in CRC and matched adjacent normal colorectal mucosa tissues. b Kaplan–Meier survival analysis of CRC patients with WTX high or WTX low expression. c Imunoblotting (IB) analyzes the WTX expression in human CRC tumor (T) and matched adjacent normal mucosa (N) tissues. d qRT-PCR analyzes WTX mRNA level in human CRC tumor and matched adjacent normal mucosa tissues. **p = 0.0023, mean ± SEM. Two-tailed Student’s t-test. e IB analyzes WTX expression in CRC cell lines. f, g IB analyzes WTX expression in indicated cell lines. h, i Transwell analyzes the migration of the indicated cell lines. Scale bars, 200 μm. j–m Colony formation analysis of the indicated cell lines. ***p < 0.001, mean ± SEM. Two-tailed Student’s t-test. n, o Gross and H&E staining observe the CRC orthotopic tumors and liver metastasis in indicated cell lines. Each arrow pointed a tumor mass. Scale bars, 20 μm, n = 11 (n) and n = 8 (o)