Fig. 3.
The CLPNR82B09s control head cast response to light. a Inhibiting CLPNR82B09s abolishes larval avoidance to white light at 550 lux (23.3 μW/mm2). b Knocking down RDL expression in CLPNR82B09s enhances larval avoidance to white light at 250 lux (11.7 μW/mm2). c–d Optogenetic stimulation of CLPNR82B09s evokes larval head cast. d is the statistics of head cast sizes in c. The pink bar indicates the period of optogenetic stimulation. e–g Knocking down RDL expression in CLPNR82B09s increases both amplitude and probability of CLPNR82B09s’ response to light in calcium imaging. The blue bar in e indicates 470 nm light stimulation at intensity of 10.58 μW/mm2. f is the statistics of peak responses in e. h–i Blocking clk-LexA neurons with TNTG does not affect the amplitude (h), but undermines the probability of CLPNR82B09s’ response to light in calcium imaging (i). One second 470 nm light stimulation at intensity of 10.58 μW/mm2 was used. In e–i, R82B09>GCAMP6m is the short for UAS-GCAMP6m/+;R82B09-Gal4/+. R82B09>GCAMP6m+RDL-RNAi is the short for UAS-GCAMP6m/UAS-RDL-RNAi;R82B09-Gal4/+. clk>TNTG;R82B09>GCAMP6m is the short for clk-LexA/UAS-GCAMP6m; R82B09-Gal4/LexAop-TNTG. Numbers above columns in g and i indicate sample sizes. n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s post hoc test in a–b, d, and f, t-test in h, fisher’s exact test in g and i. Error bars, SEMs. Source data of a–i are provided as a source data file