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. 2019 Jan 10;9:54. doi: 10.1038/s41598-018-37185-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the SLy1 SAM_wt monomer-dimer equilibrium. (a) Sedimentation equilibrium experiments were performed using samples with different SAM_wt concentrations (60, 120, and 300 µM) at different speeds. Data were fit globally with a monomer-dimer model. The upper panel shows concentration profiles recorded after establishment of equilibrium between sedimentation and back diffusion and the calculated concentration distributions (red lines) based on a monomer-dimer equilibrium model. The global fit gives a K_d = 117 (33, 423) μM. The lower panel shows the residuals of the fit. (b) Binding isotherm from microscale thermophoresis data. The thermophoresis response of fluorescent labelled SAM_wt is dependent on the total concentration of SAM_wt. The experimental data were fit to a monomer-dimer equilibrium model (solid line) with a K_d of (153 ± 25) μM.