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. 2019 Jan 10;10(1):25. doi: 10.1038/s41419-018-1242-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunoblotting of the expression of CYP24A1 in HA, HAC, NHA, and HASP normal human astrocytes, U87MG, U251, and T98G glioma cell lines and U87MG-SLC, U251-SLC, and GSC5 stem cell-like glioma cells. b The relative CYP24A1 protein levels in seven normal brain tissues and in 83 glioma tissues (12 grade II, 24 grade III, and 47 grade IV glioma tissues); actin was used as a control. The relative intensity values of CYP24A1/actin were analyzed by Image J software. c Immunoblotting of the expression of CYP24A1 in U87MG-SLC, U251-SLC, GSC2, and GSC5 cells under pH 7.4 or pH 6.8 conditions. d Immunofluorescence analysis of CYP24A1 (green) and carbonic anhydrase IX (CA IX, red) merged with nuclear DAPI staining (blue) in xenografts developed from U251, GSC2, and GSC5 cells (bar = 400 μm, left; bar = 100 μm, right). e Quantification of endogenous 1α,25(OH)₂D₃ in pH 7.4-treated and pH 6.8-treated GSC2 cells. The values shown are the means ± SD of at least three independent experiments (***P < 0.001, Student’s t-test)