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. 2019 Jan 10;9:22. doi: 10.1038/s41598-018-37063-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Newly formed bone tissue (NFBT) in tooth extraction site and non-vital bone tissue (NVBT) in tissues adjacent to tooth extraction site. (A,B) percentage of NFBT (A) and NVBT (B) in the different experimental groups at 28 postoperative days. (C–J) Photomicrographs showing percentage of NFBT in extraction site previously occupied by mesial root of first molar in groups (C,D,G,H) and NVBT in areas adjacent to extraction site (E,F,I,J) in VEH (C,E), VEH-PRP (D,F), ZOL (G,I) and ZOL-PRP (H,J). Symbols: bt, bone tissue; black arrows, osteocytes; ct, connective tissue; ii, inflammatory infiltrate; nvb, non-vital bone; white arrows, empty lacunae or occupied by necrotic remains of osteocytes; ^†Statistically significant difference in relation to VEH; ^‡Statistically significant difference in relation to VEH-PRP; ^¶Statistically significant difference in relation to ZOL. Staining: HE. Original magnification: (C,D,G,H) 100×; (E,F,I,J) 250×. Scale bars: (C,D,G,H) 250 μm; (E,F,I,J) 100 μm.