Fig. 8. miR-1254 inhibited EMT and negatively regulated PI3K/AKT signaling pathway in GC cells by downregulation of Smurf1.
a Western blot showed the change of biomarkers, such as E-cadherin, Vimentin, N-cadherin in EMT and AKT, p-Akt c-Myc, ZEB1 in cells transfected with miR-NC, miR-1254-mimics, and miR-1254-inhibitor. b The expression of these proteins were examined by western blot after the restoration of Smurf1. *p < 0.05; **p < 0.01; ***p < 0.001. The data expressed as the mean ± SD