Fig. 1. Expression and significance of FAM171A1 in breast cancer cells and tumors.

a Western blot analysis for FAM171A1 in a panel of cell lines. b, c qRT-PCR analysis of FAM171A1 and ERα in breast cancer cell lines. Unpaired two-tailed t-test; *p = 0.033 for FAM171A1; **p = 0.002 for ERα. d Boxplots showing mRNA expression of FAM171A1 among TNBC and non-TNBC tumor samples in three Oncomine datasets: TCGA, Curtis, and Kao’s breast; datasets ordered by overexpression Gene Rank-top 10%, a threshold at p value = 1E−4. e Kaplan–Meier survival curve analyses of FAM171A1 in patients with breast cancer in GSE3143 (n = 158) and Breast Invasive Carcinoma TCGA (n = 502), datasets stratified based on high (red) and low (blue) in case of GSE3143 dataset and high (red) and low (green) in case of Breast Invasive Carcinoma TCGA; and *p < 0.002, log-rank test. f Overall survival curve of breast cancer patients with hsa-miR590-5p expression in METABRIC (n = 1262) and GSE19783 (n = 101), datasets stratified based on high (black) and low (red); log-rank test. All experiments were performed in biological replicates and three technical replicates with similar results