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. 2019 Jan 9;8(1):5. doi: 10.1038/s41389-018-0113-z

Fig. 4. Effect of FAM171A1 depletion on the growth of breast cancer cells.

Fig. 4

a qRT-PCR and Western blot analyses of FAM171A1 mRNA and protein upon silencing in MDA-MB-231. b, c Anchorage-independent colony formation assay showing a decreased proliferation of MDA-MB231 cells upon FAM171A1 knockdown. d, e Representative image of the tumorspheres formed by MDA-MB-231 vs silenced MDA-MB-231 cells; scale bar: 100 µm; quantification on the top. f Status of FAM171A1 mRNA and protein in SUM149-vector and SUM149-shFAM171A1 stable cell line. g, h Anchorage-independent colony formation assay showing a decreased proliferation of SUM149 cells upon stable knockdown of FAM171A1; quantification on the top. i, j Representative image of the tumorspheres formed by SUM149 vector vs. SUM149-shFAM171A1 stable cells; scale bar: 25 µm, quantification on top; ***p-value < 0.0003; **p-value < 0.005; *p-value < 0.05; unpaired two-tailed t-test. All experiments were performed three times and with three technical replicates where needed