Fig. 5. Effect of FAM171A1 overexpression on the growth of breast cancer cells.

a Western blot analysis of FAM171A1 expression in MCF-7 stably overexpressing FAM171A1. b, c Representative images of tumorspheres formed by the indicated cells (below); scale bar: 50 μm, quantification on top; **p-value = <0.005, unpaired two-tailed t-test. d Western blot analysis of FAM171A1 in T47D stably expressing FAM171A1. e, f Representative images of tumorspheres formed by the indicated cells (below); scale bar: 50 μm, quantification on top; **p-value < 0.005, unpaired two-tailed t-test. g Reduction in the levels of FAM171A1 expression upon miR-590-5p mimic treatment in MDA-MB-231 cells. All experiments were performed three times with similar results