Table 1.
Gene name | Primers used |
---|---|
FAM171A1 | Forward 5′-CCTCGCGTTCATTTCCAGAG-3′ Reverse 5′-GGCGTTCCATTACTGCTCAG-3′ |
ESR1 (ERα) | Forward 5′-CCACCAACCAGTGCACCATT-3′ Reverse 5′-GGTCTTTTCGTATCCCACCTTTC-3′ |
HER2 | Forward 5′-AACTGCACCCACTCCTGTGT-3′ Reverse 5′-TGATGAGGATCCCAAAGACC-3′ |
GAPDH | Forward 5′-CACCAGGGCTGCTTTTAACTCTGGTA-3′ Reverse 5′-CCTTGACGGTGCCATGGAATTTGC-3′ |
FAM(ChIP)-ERα1 | Forward 5′-CCCAGCCAAGTCTGGAGGT-3′ Reverse 5′-GGGCACAACTGAGCCTAGAAG-3′ |
FAM(ChIP)-ERα2 | Forward 5′-GAGGGTCTTCTTTGCTGTGC-3′ Reverse 5′-ACCAGGTGCCATCCTAATGT-3′ |
PS2(ChIP) | Forward 5′-TTAGCTTAGGCCTAGACGGAATGG-3′ Reverse 5′-GACGACATGTGGTGAGGTCATCTT-3′ |
CYCLIN-D(ChIP) | Forward 5′-CATTCAGAGGTGTGTTTCTCCC-3′ Reverse 5′-CTCAGCGACTGCATCTTCTTTC-3′ |
FAM-CHIP-ERα3 | Forward 5′-GAGGGTCTTCTTTGCTGTGC-3′ Reverse 5′-TCAGGAGGAATGGTACTTGGA-3′ |
Snail | Forward 5′-AATCGGAAGCCTAACTACAGCGAG-3′ Reverse 5′-CCTTGGCCTCAGAGAGCTGG-3′ |
Slug | Forward 5′-AGATGCATATTCGGACCCAC-3′ Reverse 5′-CCTCATGTTTGTGCAGGAGA-3′ |
Vimentin | Forward 5′-GACAATGCGTCTCTGGCACGTCTT-3′ Reverse 5′-TCCTCCGCCTCCTGCAGGTTCTT-3′ |
E-cadherin | Forward 5′-GAAGGTGACAGAGCCTCTGGAT-3′ Reverse 5′-GATCGGTTACCGTGATCAAAATC-3′ |
N-cadherin | Forward 5′-ACAGTGGCCACCTACAAAGG-3′ Reverse 5′-CCGAGATGGGGTTGATAATG-3′ |
ZEB1 | Forward 5′-GGCAGAGAATGAGGGAGAAG-3′ Reverse 5′-CTTCAGACACTTGCTCACTACTC-3′ |
Pre-miR-590 | Forward 5′-GGCTATCCTCTCAGAGTGACATTT-3′ Reverse 5′-GCTTTATCAGGTTATGTTGCATGGT-3′ |
miR-590-5p | Forward 5′-AAAGGATCCGTGGGTGAGTATGGGAGGGA-3′ Reverse 5′-AAAAAGCTTGCCAAGCTAAGCCAAGGGTA-3′ |
miR-590 promoter | Forward 5′-CTTTATGCTGACCTGCTTG-3′ Reverse 5′-TCTCCTTGTTGCCTGATT-3′ |
miR-590(ChIP) | Forward 5′-TCAAGCGATTCTCCTGC-3′ Reverse 5′-AAGCGTCGTTTACTCTGC-3′ |
SG6K(ChIP) | Forward 5′-TGCAAGGAGAACATTGAACACAA-3′ Reverse 5′-CCGGCGTGCCACAGA-3′ |
DNAMT3A(ChIP) | Forward 5′-AGGAACCTAGAGCCCTGAGC-3′ Reverse 5′-GTGAGTTCCCCGTACCTTGA-3′ |
FAM −3′-UTR | Forward 5′-AAACTCGAGAGACCCACCTGACTGTGGAATATAA-3′ Reverse 5′-AAAGCGGCCCCTTTTCTTCAGAGTTTATTTTGAA-3′ |
U6 snRNA | Forward 5′-CTCGCTTCGGCAGCACA-3′ Reverse 5′-AACGCTTCACGAATTTGCGT-3′ |