Fig. 1.

Pioglitazone reduces carcinogenesis in the rat DEN model. At the time of sacrifice, a overall body weight and b liver weight/body weight ratios were measured. c Representative gross pictures (top panel), representative H&E staining (× 5 original magnification, middle panel), and immunohistochemical proliferating cell nuclear antigen (PCNA) staining (× 5 original magnification, bottom panel) are shown. d Total surface tumors were counted and separated by size as e < 8 mm and f > 8 mm. g PCNA staining was quantified using image J software. # p < 0.05 and ## p < 0.01 compared to PBS. * p < 0.05 and ** p < 0.01 compared to DEN