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. 2018 Oct 26;23(1):101–111. doi: 10.1007/s11605-018-4004-6

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Pioglitazone reduces fibrosis/cirrhosis in the rat DEN model. a Sirius red staining (× 10 original magnification, top panel) and α-SMA immunofluorescence staining (× 10 original magnification, bottom panel) were performed to assess fibrosis and hepatic stellate cell activation, respectively. b Morphometric assessment of the collagen proportional area (CPA) on sirius red-stained slides. Gene expression of fibrosis markers including cActa2, dCol1a1, eTgf-b1, and fTimp1. # p < 0.05 and ## p < 0.01compared to PBS. * p < 0.05 and ** p < 0.01 compared to DEN