Fig. 6.

Pioglitazone reduces inflammation and fibrosis in the mouse DEN + CDAHFD model. Gene expression of several pro-inflammatory markers including aIl6, bCcl2, cCd68, and dIfn-y was measured. e Sirius red staining was performed to assess fibrosis and the f collagen proportional area (CPA) was calculated. Fibrotic gene expression including gActa2, hCol1a1, iTgfb-1, and jTimp1 was measured. k The non-alcoholic fatty liver disease (NAFLD) activity score (NAS) and l the NASH fibrosis score were scored by a blinded liver pathologist. m Lipid vacuolization (LV) was morphometrically calculated using image J software. # p < 0.05 and ## p < 0.01 compared to standard chow. * p < 0.05 and ** p < 0.01 compared to DEN+CDAHFD