Fig. 5.

ISH staining of CB₁ mRNA in ileum of healthy (a–d) and LPS-treated (e) mice. Strong CB₁ mRNA ISH staining (brown, DAB) is seen in myenteric (asterisk) and submucosal ganglia (double asterisk) in the ileal wall of healthy mice (a). There is only low expression of CB₁ mRNA (arrows) in the epithelium (bep) and the lamina propria (blp). High magnification of lymph follicles shows CB₁ gene expression in healthy animals (c). High levels of CB₁ mRNA (FastRed) are detected in isolated cells of the epithelium, probably neuroendocrine cells (arrowheads), from healthy (d) as well as LPS-treated (e) mice. Only marginal staining of CB₁ mRNA is found in CD3⁺ cells (d, purple, VIP; arrows) that is even less in the ileum of LPS-treated animals (e). Quantification of CB₁/CD3⁺ double staining is shown in f. Calibration bar in a: 50 µm; calibration bar in b, c: 10 µm; calibration bar in d, e: 20 µm; cm circular muscle layer, ep epithelium, lp lamina propria, lm longitudinal muscle layer; *, myenteric ganglion; **, submucosal ganglion