Fig. 6.

ISH staining of CB₂ mRNA in ileum of healthy (a, b, d, f) and LPS-treated (e, g) mice. Healthy mice show very low gene expression of CB₂ (brown, DAB) in myenteric ganglia of the ileum (a marked by asterisk; arrows point at mRNA staining). Compared to myenteric ganglia, cells in lymph follicles show widespread CB₂ gene expression, some located in CD3⁺ cells (b arrows). The CB₂ gene is also expressed in lamina propria CD3⁺ cells of healthy animals, although at a low level (d arrows point at expression in representative cells). Cells often line up underneath the epithelium (ep; e). Also CD3 negative cells expressing the CB₂ gene are visible In the lamina propria (lp) (e arrowhead). In addition, cells stained for F4/80 show CB₂ mRNA in healthy (f) and LPS-treated (g) mice. There is only marginal expression of CB₂ in epithelial cells even after LPS treatment (g; arrowhead). Quantification of CB₂ mRNA levels in CD3⁺ cells is shown in c, indicating that CB₂ gene expression is not significantly altered after LPS treatment. Calibration bars: 10 µm; ep epithelium, lp lamina propria, *, myenteric ganglion