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Effect of MnII on the growth of Roseobacter sp. AzwK-3b in the defined growth medium (Table 1). The concentrations of manganese were 0 μM (black), 200 μM (red), and 500 μM (dark green), with no growth (zero line) in the respective noninoculated controls (blue, magenta, and light blue). Cultures were grown in a 96-well plate (200-μl culture), with shaking, and absorbance measurements were taken every 10 min (see Materials and Methods).
