FIG 3.

Growth of Roseobacter sp. AzwK-3b cultures in the defined growth medium supplemented with sodium nitrite. Media were prepared without (A) or with (B) 200 μM manganese chloride (Mn^IICl₂). Nitrite concentrations were 0 mM (black), 0.25 mM (red), 0.5 mM (green), 1 mM (dark blue), and 2.5 mM (light blue). All conditions were tested in triplicate, and the growth curves represent averages and their standard deviations (see Materials and Methods).