. 2019 Jan 9;85(2):e02129-18. doi: 10.1128/AEM.02129-18

TABLE 1.

Detailed composition of the defined AzwK-3b growth medium, ASW_m^a

Compound	Concn
Base salts (1× AzwK-3b medium)
Sodium chloride (NaCl) (mM)	200
Ammonium chloride (NH₄Cl) (mM)	8.82
Potassium chloride (KCl) (mM)	6.71
Di-potassium hydrogen phosphate (KH₂PO₄) (μM)	131
Magnesium sulfate (MgSO₄) (mM)	14.2
Magnesium chloride (MgCl₂) (mM)	9.84
Calcium chloride (CaCl₂) (mM)	3
Tris (mM)	1.1
pH of the medium	8
Trace metal solution (1,000×)
Copper chloride (CuCl₂) (μM)	32
Zinc sulfate (ZnSO₄) (μM)	765
Cobalt chloride (CoCl₂) (μM)	169
Sodium molybdate (Na₂MoO₄) (mM)	1.65
Boric acid (H₃BO₃) (mM)	46.3
Nickel chloride (NiCl₂) (mM)	4.2
Sodium tungstate (Na₂WoO₄) (μM)	243
Sodium selenite (Na₂SeO₃) (μM)	228
Additional (1,000×) supplement solutions
Iron chloride (FeCl₃; prepared in 10 mM HCl, balanced with extra 10 mM NaOH solution) (mM)	10.4
EDTA (pH 8.0; sodium salt) (mM)	1.34
Manganese chloride (MnCl₂, only added where desired) (mM)	200
Vitamin supplement (1,000×)
Biotin (μM)	82
Pyridoxine hydrochloride (μM)	484
Thiamine hydrochloride (μM)	148
Riboflavin (μM)	133
Nicotinic acid (μM)	406

The medium was developed starting from artificial seawater (ASW) (35), with extra trace metals taken from references 9 and 94 and a 5-vitamin solution identified starting from Wolfe’s vitamin mixture (95).