FIG 2.

Hydrolytic activity on complex polysaccharide substrates (average values from two duplicate experiments) of proteins collected from growth medium of C. pinensis supplemented with different carbohydrate carbon sources. Glc, glucose; CMC, carboxymethyl-cellulose; MLG, barley mixed-linkage β-glucan (MLG); ABG, linear A. bisporus β-glucan; CUR, curdlan; SCL, scleroglucan; KGM, konjac glucomannan; WAX, wheat arabinoxylan. 1 U = 1 mmol reducing sugar (glucose equivalents) released/min. We previously showed that glucose-grown cultures do not secrete any detectable polysaccharide-degrading enzymes (15). Activity data shown for KGM cultures were also originally presented in reference 15. Table S4 shows these data in numerical form, including standard deviation error values.