TABLE 2.
Primers used in this study
| Primer name and purpose | Sequencea (5′–3′) |
|---|---|
| Modification of pTrc99A to pTrc100 | |
| H_5907_F | TCTAGAGGTACCGGATCCATGTGGCAGTTTAATGACCTGCA |
| H_5907_R | GTCGACGGATCCTTAATCCAGACGGATAAATG |
| Open reading frame of effectors | |
| WHH_F | GAGCTCGGTACCATGGCGAATATCAGAAAGCAAAGCG |
| WHH_R | CTGCAGGTCGACTTAATAATCCTCGCTACGCCACATCG |
| Phos_F | GAGCTCGGTACCATGCCAGGTGCTGCACGTTTAG |
| Phos_R | CTGCAGGTCGACTTATATTCCCCTGAATGTATTGG |
| AHH_F | GAGCTCGGTACCATGGACTGGGTTGATCCGTTTGGGTTGGC |
| AHH_R | CTGCAGGGTACCTCAATATTTAAACGTCCCTGAAACAAGTG |
| D123_F | GTCGACGGTACCATGTATTCTGAACATAAGGCGAC |
| D123_R | CTGCAGAAGCTTCTATAATAAATAGGGTCTTCTTTG |
| Immunity gene for AHH nuclease | |
| AHH-I_F | GAGCTCGGTACCATGAACATTTACACGCTAGAAGC |
| AHH-I_R | CTGCAGGTCGACCTAATCTGATACTCCAACC |
a
Cut sites are underlined.