TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant characteristics or sequencea | Reference |
|---|---|---|
| Strains | ||
| Acetobacter fabarum | ||
| DsW_054 | From D. suzukii gut | 44 |
| 108A11 | Tn5::ppdK | This study |
| 108A11c | 108A11 plus pMQ97-Tc-ppdK | This study |
| 12A2 | Tn5::ppdK | 41 |
| 12A2c | 12A2 plus pMQ97-Tc-ppdK | This study |
| 66G2 | Tn5::ppdK | 41 |
| ldh1 (10B7) | Tn5::ldh | 41 |
| ldh2 (92G1) | Tn5::ldh | 41 |
| adh1 (21G4) | Tn5::adh | 41 |
| adh2 (5F1) | Tn5::adh | 41 |
| als (36G1) | Tn5::als | 41 |
| aldC (26G11) | Tn5::aldC | 41 |
| pdc (63F5) | Tn5::pdc | 41 |
| Acetobacter tropicalis DmCS_007 | From D. melanogaster gut | 49 |
| Lactobacillus brevis DmCS_003 | From D. melanogaster gut | 49 |
| Escherichia coli S17-1 (λ-pir) | Conjugation donor | 67 |
| Saccharomyces cerevisiae InvSc1 | URA− | 66 |
| Plasmids | ||
| pRL27 | Tn5 delivery vector; Kmr | 64 |
| pKO3 | Tcr | 65 |
| pMQ97 | RK2 ori, URA3, 2 μm | 66 |
| pMQ97-Tc | Tcr | This study |
| pMQ97-Tc-ppdK | ppdK with native promoter | This study |
| Primers | ||
| Extsx | GACAACAAGCCAGGGATG | 68 |
| Intsx | CGCACTGAGAAGCCCTTAGAGC | 68 |
| Arb1 | GGCCACGCGTCGACTAGTACN10GATAT | 65 |
| Arb2 | GGCCACGCGTCGACTAGTAC | 65 |
| Arb6 | GGCCACGCGTCGACTAGTACN10ACGCC | 65 |
| Tc_Fwd | GTGAATCCGTTAGCGAGGTGC | This study |
| Tc_Rev | CCGATCTCGGCTTGAACGAATTG | This study |
| ppdK_YC_Fwd | CGTAACAAACGGATAGAACCAC | This study |
| ppdK_YC_Rev | CGGAAACGAGGTGGAGAGTAAG | This study |
a
Kmr, kanamycin resistance; Tcr, tetracycline resistance.